Antioxidants and Free Radicals

Oct 13, 2025 by pynkocean.com 138 views

Antioxidant Activity Test in Yellow Dragon Fruit Juice (HAW.) Britton & Rose) with the DPPH Government Method (1,1-diphenyl 2-Picrylhydrazil)

Discussion Category: Undergraduate Theses, Education, Journal, Thesis, University, Student, Campus

Test of Antioxidant Activity on Yellow Dragon Fruit Juice (Selenicereus Megalanthus)

Yellow dragon fruit, or known as scientific name selenicereus megalanthus, is one type of fruit that is rich in nutritional and has great potential as a source of antioxidants. The high antioxidant content in yellow dragon fruit makes it an excellent choice for those looking to improve their overall health. This study aims to test the antioxidant activity of yellow dragon fruit juice using the DPPH free radicals (1,1-diphenyl-2-picrylhydrazyl method. This method utilizes the visible spectrophotometer to measure the color changes that occur in the solution when the DPPH radical is captured by antioxidant compounds.

Antioxidants and Free Radicals

Antioxidants are compounds that have the ability to provide electrons and neutralize free radicals. Free radicals are molecules that have unpaired electrons, which can damage body cells and contribute to various diseases, including cancer and heart disease. Therefore, consumption of foods that are rich in antioxidants are very important to maintain a healthy body. Yellow dragon fruit contains vitamin C, vitamin E, vitamin A, and polyphenol compounds that are known to play an active role as antioxidants. By consuming yellow dragon fruit juice, we not only get a fresh and refreshing taste, but also significant health benefits.

Research Methods

In this study, the antioxidant activity test was carried out by measuring the percentage of DPPH devices at various concentrations of yellow dragon fruit juice, which is 50 μg/ml; 100 μg/ml; 150 μg/ml; and 200 μg/ml. Measurements are made at a maximum wavelength of 516 Nm, where the results show the percent of the actors as follows:

At a concentration of 50 μg/ml: 28.12%
At a concentration of 100 μg/ml: 53.36%
At a concentration of 150 μg/ml: 75.78%
At a concentration of 200 μg/ml: 92.54%

As a comparison, the testing of antioxidant activity from vitamin C is also carried out at a concentration of 2 μg/ml; 4 μg/ml; 6 μg/ml; and 8 μg/ml, with the results of the percentage as follows:

At a concentration of 2 μg/ml: 20.723%
At a concentration of 4 μg/ml: 45.956%
At a concentration of 6 μg/ml: 75.067%
At a concentration of 8 μg/ml: 93.733%

Results and Analysis

From the results of the study, it can be concluded that yellow dragon fruit juice has a strong antioxidant activity with an IC50 value of 100,0859 μg/ml. This shows that yellow dragon fruit juice is effective in capturing free radicals. As a comparison, the antioxidant activity of vitamin C has a much lower IC50 value, which is 4.24 μg/ml. This shows that although yellow dragon fruit juice has strong antioxidant activity, vitamin C remains one of the more efficient sources of antioxidants.

These results underline the importance of further research on the potential of yellow dragon fruit as a source of natural antioxidants. Inserting yellow dragon fruit juice in a daily diet can be an effective way to fight free radicals and improve overall health.

The Benefits of Yellow Dragon Fruit Juice

With all its benefits, yellow dragon fruit juice not only offers delicious taste, but also provides additional protection for the health of the body, making it a suitable choice for regular consumption. The high antioxidant content in yellow dragon fruit juice makes it an excellent choice for those looking to improve their overall health. By incorporating yellow dragon fruit juice into your daily diet, you can enjoy a range of health benefits, including:

Reducing the risk of chronic diseases: Yellow dragon fruit juice is rich in antioxidants, which can help to reduce the risk of chronic diseases such as heart disease, cancer, and Alzheimer's disease.
Improving cognitive function: The antioxidants in yellow dragon fruit juice can help to improve cognitive function and reduce the risk of age-related cognitive decline.
Supporting immune function: Yellow dragon fruit juice is rich in vitamin C, which can help to support immune function and reduce the risk of illness.
Promoting healthy skin: The antioxidants in yellow dragon fruit juice can help to promote healthy skin and reduce the risk of skin damage.

Conclusion

In conclusion, the results of this study demonstrate the strong antioxidant activity of yellow dragon fruit juice. The high antioxidant content in yellow dragon fruit juice makes it an excellent choice for those looking to improve their overall health. By incorporating yellow dragon fruit juice into your daily diet, you can enjoy a range of health benefits, including reducing the risk of chronic diseases, improving cognitive function, supporting immune function, and promoting healthy skin. Further research is needed to fully understand the potential of yellow dragon fruit as a source of natural antioxidants.
Frequently Asked Questions (FAQs) about Antioxidant Activity Test in Yellow Dragon Fruit Juice

Q: What is the purpose of the antioxidant activity test in yellow dragon fruit juice?

A: The purpose of the antioxidant activity test in yellow dragon fruit juice is to measure the ability of the juice to capture free radicals and neutralize them, which can help to reduce the risk of chronic diseases.

Q: What is the DPPH method used in the antioxidant activity test?

A: The DPPH method is a widely used method for measuring antioxidant activity, which involves measuring the ability of a compound to capture free radicals and neutralize them.

Q: What are the benefits of consuming yellow dragon fruit juice?

A: Consuming yellow dragon fruit juice can provide a range of health benefits, including reducing the risk of chronic diseases, improving cognitive function, supporting immune function, and promoting healthy skin.

Q: How does yellow dragon fruit juice compare to vitamin C in terms of antioxidant activity?

A: The antioxidant activity of yellow dragon fruit juice is strong, with an IC50 value of 100,0859 μg/ml, which is higher than that of vitamin C, which has an IC50 value of 4.24 μg/ml.

Q: What are the potential health benefits of consuming yellow dragon fruit juice?

A: The potential health benefits of consuming yellow dragon fruit juice include reducing the risk of chronic diseases, improving cognitive function, supporting immune function, and promoting healthy skin.

Q: Is yellow dragon fruit juice a suitable choice for regular consumption?

A: Yes, yellow dragon fruit juice is a suitable choice for regular consumption due to its high antioxidant content and potential health benefits.

Q: Can yellow dragon fruit juice be used as a natural source of antioxidants?

A: Yes, yellow dragon fruit juice can be used as a natural source of antioxidants, which can help to reduce the risk of chronic diseases and promote overall health.

Q: What are the potential risks associated with consuming yellow dragon fruit juice?

A: There are no known risks associated with consuming yellow dragon fruit juice, but it is always recommended to consult with a healthcare professional before making any significant changes to your diet.

Q: How can I incorporate yellow dragon fruit juice into my daily diet?

A: You can incorporate yellow dragon fruit juice into your daily diet by drinking it as a beverage, using it as a base for smoothies, or adding it to your favorite recipes.

Q: Can I grow my own yellow dragon fruit plant?

A: Yes, you can grow your own yellow dragon fruit plant, which can provide you with a steady supply of fresh fruit and juice.

Q: Where can I purchase yellow dragon fruit juice?

A: You can purchase yellow dragon fruit juice at most health food stores, specialty grocery stores, or online retailers.

Q: Is yellow dragon fruit juice suitable for people with dietary restrictions?

A: Yes, yellow dragon fruit juice is suitable for people with dietary restrictions, including vegetarians, vegans, and those with gluten intolerance.

Q: Can I use yellow dragon fruit juice as a natural remedy for any health conditions?

A: While yellow dragon fruit juice may have potential health benefits, it should not be used as a natural remedy for any health conditions without consulting with a healthcare professional first.