Formulations in health products

Oct 13, 2025 by pynkocean.com 87 views

Analysis of Chemical Compounds of Sembung Root Essential Oil (Blumea Balsamifera DC) with GC-MS and Antimicrobial and Antioxidant Activity Test

Introduction

Essential oils have been widely used in various industries, including the health and cosmetics sectors, due to their unique properties and benefits. One of the essential oils that have gained attention in recent years is the Sembung root essential oil, which is derived from the roots of the Blumea Balsamifera DC plant. This oil has been traditionally used in various cultures for its medicinal and therapeutic properties. In this study, we aimed to analyze the chemical compounds of Sembung root essential oil using gas chromatography-mass spectrometry (GC-MS) and to evaluate its antimicrobial and antioxidant activities.

Materials and Methods

The Sembung root essential oil was isolated from the roots of the Blumea Balsamifera DC plant using the hydrodistillation method. The oil was then analyzed using GC-MS to identify its chemical compounds. The antimicrobial activity of the oil was evaluated using the agar disc diffusion method against four types of microbes, namely Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Candida Albicans. The antioxidant activity of the oil was evaluated using the DPPH method (2,2-diphenyl-1-picrylhydrazyl).

Results

The results of the GC-MS analysis showed that the Sembung root essential oil contains 21 chemical compounds, of which 14 are the main components with content above 1%. The main components include Propane, 2-Ethoxy; 1,3,6-Octatriene, 3,7-Dimethyl (occimena); Thujopsen-13; Thymohydroquinone Dimethyl Ether; 2-Allyl-1,4-Dimethoxy-3-methyl-Benzene; Thymol; Neryl acetat; 1,5-Dimethyl-1-Vinil-4-Hexenyl-3-methil buttanoate; Geranyl Pentanoate; 5-oxatricyclo [8.2.0.0 (4,6)] Dodecane; 1,2-trimethyl-9-methylene; and Tridecane.

The antimicrobial test results showed that the Sembung root essential oil has significant activity against four types of microbes, namely Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Candida Albicans. This positive result indicates the potential of the essential oil as an antimicrobial agent that can be used in the treatment or prevention of infections caused by these microbes.

The antioxidant activity test results showed that the Sembung root essential oil has good antioxidant activity with a concentration of 12.1 ppm. This activity indicates that the essential oil from the Sembung root can function as a natural antioxidant source.

Discussion

Essential oils have been widely used in various industries due to their unique properties and benefits. The Sembung root essential oil, which is derived from the roots of the Blumea Balsamifera DC plant, has been traditionally used in various cultures for its medicinal and therapeutic properties. In this study, we aimed to analyze the chemical compounds of Sembung root essential oil using GC-MS and to evaluate its antimicrobial and antioxidant activities.

Conclusion

Based on the results of the chemical analysis and antimicrobial and antioxidant activity tests, the Sembung root essential oil has good potential to be used in the health field, both as an antimicrobial and as a source of natural antioxidants. Further research can be conducted to explore various other potentials of this essential oil, including formulations in health or cosmetics products. This will certainly provide added value in the utilization of traditional medicinal plants in the modern era.

References

[1] Blumea Balsamifera DC: A plant species that has been traditionally used in various cultures for its medicinal and therapeutic properties.
[2] GC-MS: A combined method of gas chromatography and mass spectrometry used to analyze the chemical compounds of essential oils.
[3] Antimicrobial activity: The ability of essential oils to inhibit the growth of microorganisms, such as bacteria and fungi.
[4] Antioxidant activity: The ability of essential oils to prevent or reduce the damage caused by free radicals, which can lead to various degenerative diseases.

Future Directions

Further research can be conducted to explore various other potentials of the Sembung root essential oil, including:

Formulations in health products: The essential oil can be formulated into various health products, such as creams, ointments, and capsules, to provide antimicrobial and antioxidant benefits.
Cosmetics products: The essential oil can be used in various cosmetics products, such as soaps, shampoos, and lotions, to provide antimicrobial and antioxidant benefits.
Pharmaceutical applications: The essential oil can be used as a natural antimicrobial and antioxidant agent in various pharmaceutical applications, such as wound care and skin care products.

Conclusion

In conclusion, the Sembung root essential oil has good potential to be used in the health field, both as an antimicrobial and as a source of natural antioxidants. Further research can be conducted to explore various other potentials of this essential oil, including formulations in health or cosmetics products. This will certainly provide added value in the utilization of traditional medicinal plants in the modern era.
Q&A: Analysis of Chemical Compounds of Sembung Root Essential Oil (Blumea Balsamifera DC) with GC-MS and Antimicrobial and Antioxidant Activity Test

Q: What is Sembung root essential oil?

A: Sembung root essential oil is a type of essential oil that is derived from the roots of the Blumea Balsamifera DC plant. It has been traditionally used in various cultures for its medicinal and therapeutic properties.

Q: What is GC-MS?

A: GC-MS stands for gas chromatography-mass spectrometry, which is a combined method used to analyze the chemical compounds of essential oils. It helps to identify the different components of the essential oil and their concentrations.

Q: What are the main components of Sembung root essential oil?

A: The main components of Sembung root essential oil include Propane, 2-Ethoxy; 1,3,6-Octatriene, 3,7-Dimethyl (occimena); Thujopsen-13; Thymohydroquinone Dimethyl Ether; 2-Allyl-1,4-Dimethoxy-3-methyl-Benzene; Thymol; Neryl acetat; 1,5-Dimethyl-1-Vinil-4-Hexenyl-3-methil buttanoate; Geranyl Pentanoate; 5-oxatricyclo [8.2.0.0 (4,6)] Dodecane; 1,2-trimethyl-9-methylene; and Tridecane.

Q: What is the antimicrobial activity of Sembung root essential oil?

A: The antimicrobial activity of Sembung root essential oil was evaluated using the agar disc diffusion method against four types of microbes, namely Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Candida Albicans. The results showed that the essential oil has significant activity against these microbes.

Q: What is the antioxidant activity of Sembung root essential oil?

A: The antioxidant activity of Sembung root essential oil was evaluated using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The results showed that the essential oil has good antioxidant activity with a concentration of 12.1 ppm.

Q: What are the potential applications of Sembung root essential oil?

A: Sembung root essential oil has good potential to be used in the health field, both as an antimicrobial and as a source of natural antioxidants. It can be formulated into various health products, such as creams, ointments, and capsules, to provide antimicrobial and antioxidant benefits.

Q: Can Sembung root essential oil be used in cosmetics products?

A: Yes, Sembung root essential oil can be used in various cosmetics products, such as soaps, shampoos, and lotions, to provide antimicrobial and antioxidant benefits.

Q: What are the benefits of using Sembung root essential oil?

A: The benefits of using Sembung root essential oil include its antimicrobial and antioxidant properties, which can help to prevent or reduce the damage caused by free radicals and microorganisms.

Q: Is Sembung root essential oil safe to use?

A: Sembung root essential oil is generally considered safe to use, but it is recommended to consult with a healthcare professional before using it, especially if you have any underlying medical conditions or allergies.

Q: Where can I find Sembung root essential oil?

A: Sembung root essential oil can be found in various health food stores, online retailers, and specialty stores that sell essential oils.

Q: How do I use Sembung root essential oil?

A: Sembung root essential oil can be used in various ways, including topical application, inhalation, and internal use. It is recommended to consult with a healthcare professional before using it, especially if you have any underlying medical conditions or allergies.